Acetylcholinesterase [AChE] activity and isozyme pattern in normal and lithium-treated developing chick brain

Gouri, Ms Anahita (2004) Acetylcholinesterase [AChE] activity and isozyme pattern in normal and lithium-treated developing chick brain. [Thesis]

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Literature Survey: - Acetylcholinesterase [AChE] is an enzyme that terminates Acetylcholine [ACh] mediated neurotransmission. Its wide neuronal and non-neuronal cellular distribution has made it the focus of intense research. The data on tissue and species specific AChE expression, the detection of its various isoforms and its cholinergic and non-cholinergic functions has been accumulated and reviewed from a range of evolutionary diverse vertebrates and invertebrates that include insects, nematodes, fish, reptiles, birds and several mammals, among them man. Project Work: - Acetylcholinesterase [AChE], the lytic enzyme of the cholinergic system, functions in hydrolyzing the neurotransmitter Acetylcholine [ACh] and hence is used as a marker for cholinergic function. In vertebrates the protein is synthesized by a single gene and undergoes alternative splicing to give several isoforms. This enzyme and its isoforms are also involved in synaptogenesis, modulated by stages of development and differentially distributed in the brain. Not only is it known to be a marker for the developing chick brain, but is also implicated in neurodegenerative diseases like Alzheimer’s Disease. Isozyme Pattern of AChE is suggested to serve as a useful prognostic marker in neuronal degeneration. Lithium, a well-known teratogen, has been shown in our laboratory to induce apoptosis in a developing chick brain. Understanding the dynamics of AChE isoform pattern in lithium induced neural tissue damage would help elucidating the role of these isoforms in a degenerating system and add to our understanding of neurodegenerative diseases. We have therefore studied activity and isozyme pattern of AChE in lithium-treated and control 7-day old developing chick brain and report the same.

Item Type:Thesis
Additional Information:A poster on this research work was presented at the “XXVII All India Cell Biology Conference” held in January 2004 at the University of Pune.A platform presentation was delivered at the “ Joint International Neuroscience Conference” held in May 2004, at the university of Hyderabad. The author was honoured with the ‘D.M Kar - Young scientist 2004 award’. This project also received the 'Lady Tata Memorial Trust’ scholarship.
Keywords:Anahita Gouri, acetylcholinesterase, Lithium, Developing chick brain, invertebrate AChE, vertebrate AChE
Subjects:Neuroscience > Neurochemistry
ID Code:4667
Deposited By: Gouri, Ms Anahita
Deposited On:30 Dec 2005
Last Modified:11 Mar 2011 08:56

References in Article

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1. Arnon R, Silman I, Tarrab-Hazdai R. in “AChE of Schistosoma mansoni--functional correlates.Contributed in honor of Professor Hans Neurath's 90th birthday.”Protein Sci.1999 Dec;8(12):2553-61.

2. Ashwani V: Biochemical Adaption of Brain AChE during temperature acclimmation of fish Tilapia massambica Peters. Wilson College, University of Mumbai (1992).

3. Atack J., Perry E., Bonham J., and Perry R. in “Molecular forms of AChE and Butyrylcholinesterase in Human Plasma and Cerebrospinal Fluid.” Journal of Neurochemistry (1987).

4. Bocquene G, Roig A, Fournier D. in “Cholinesterases from the common oyster (Crassostrea gigas). Evidence for the presence of a soluble AChE insensitive to organophosphate and carbamate inhibitors.” FEBS Lett. 1997 May 5; 407(3): 261-6.

5. Bourguet D, Roig A, Toutant JP, Arpagaus M. in “Analysis of molecular forms and pharmacological properties of AChE in several mosquito species.” Neurochem Int. 1997 Jul; 31(1): 65-72

6. Brimijoin S., in “Molecular forms of AChE in Brain, Nerve and Muscle: Nature, Localization and Dynamics.” Progress in neurobiology Vol.21.pp. 291 to 322, 1983.

7. Camacho M, Tarrab-Hazdai R, Espinoza B, Arnon R, Agnew A in “The amount of AChE on the parasite surface reflects the differential sensitivity of schistosome species to metrifonate.” Parasitology. 1994 Feb;108 ( Pt 2):153-60.

8. Combes D, Fedon Y, Grauso M, Toutant JP, Arpagaus M. in “Four genes encode AChE in the nematodes Caenorhabditis elegans and

Caenorhabditis briggsae. cDNA sequences, genomic structures, mutations and in vivo expression.” J Mol Biol. 2000 Jul 21;300(4):727-42.

9. Combes D, Fedon Y, Toutant JP, Arpagaus M in “Multiple ace genes encoding AChE of Caenorhabditis elegans have distinct tissue expression.” Eur J Neurosci. 2003 Aug;18(3):497-512

10. Combes D, Fedon Y, Toutant JP, Arpagaus M. in “AChE genes in the nematode Caenorhabditis elegans.” Int Rev Cytol. 2001;209:207-39.

11. Corrado M.U.D, Politi H, Ognibene M, Angelini C, Trielli F, Ballarini P AND Falugi C in “Synthesis of the Signal Molecule Acetylcholine during the Developmental Cycle of Paramecium primaurelia (Protista, Ciliophora) and its possible function in conjugation. The Journal of Exp Biology204,1901–1907 (2001)

12. Cousin X, Bon S, Massoulie´J, and Bon C in “Identification of a Novel Type of Alternatively Spliced Exon from the Acetylcholinesterase Gene of Bungarus fasciatus.” THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 273, No. 16, Issue of April 17, pp. 9812–9820, 1998

13. den Besten PJ, Valk S, van Weerlee E, Nolting RF, Postma JF, Everaarts JM. In “Bioaccumulation and biomarkers in the sea star Asterias rubens (Echinodermata: Asteroidea): a North Sea field study.” Mar Environ Res. 2001 May;51(4):365-87.

14. Falugi C, Amaroli A, Evangelisti V, Viarengo A, Corrado MU in “Cholinesterase activity and effects of its inhibition by neurotoxic drugs in Dictyostelium discoideum”. Chemosphere. 2002 Jul;48(4):407-14.

15. Falugi C, Diaspro A, Angelini C, Pedrotti ML, Raimondo M, Robello M. in “Three-dimensional mapping of cholinergic molecules by confocal laser scanning microscopy in sea urchin larvae.” Micron. 2002;33(3):233-9.

16. Gao J.R., Kambhampati.S., Zhu.K.Y in “ Molecular Cloning and characterization of a greenbug(Schizaphis graminum) cDNA encoding acetylcholinesterase possibly evolved from a duplicate gene lineage.” Insect Biochemistry and Molecular Biology 32 (2002) 765-775.

17. Gimenez-Pardo C, Ros Moreno RM, De Armas-Serra C, Rodriguez-Caabeiro F in “Presence of cholinesterases in Echinococcus granulosus protoscolices.” Parasite. 2000 Mar;7(1):47-50.

18. Hamilton H.L ,Lillie’s Development of Chick: An introdustion to Embryology;Holt,Rinehart and Winston, New York;1965.

19. Harrison P. K., Falugi C., Angelini C., Whitaker M. J. in “Muscarinic signalling affects intracellular calcium concentration during the .rst cell cycle of sea urchin embryos.” Cell Calcium (2002) 284(0), 1–9

20. Hollander BA, Bennett GS in “Lithium chloride alters cytoskeletal organization in growing, but not mature, cultured chick sensory neurons.” Neurosci Res. 1991 Mar;28(3):332-42.

21. Ivanenkov VV, Korobtsov GN in “Heat stability of cholinesterase and non-specific esterases during development of hybrids of the sea urchins Stongylocentrotus droebachiensis and S. intermedius.” Ontogenez. 1976;7(4):341-9.

22. Janka Z., Szentistvanyi I., Joo F., Juhasz A., and Rimanoczy A. in “Effects of Lithium on Morphological Characteristics of Dissociated Brain Cells in Culture.” Acta Neuropathol (Berl.)46,117-121(1979).

23. Johnson G. and Moore S. in “Association of the HNK-1 epitope with the detergent-soluble G4 isoform of acetylcholinesterase from human neuroblastoma cells.” Int.J Devl Neuroscience 19 (2001) 439-445.

24. Jones AK, Bentley GN, Oliveros Parra WG, Agnew A. in “Molecular characterization of an acetylcholinesterase implicated in the regulation of glucose scavenging by the parasite Schistosoma.” FASEB J. 2002 Mar;16(3):441-3. Epub 2002 Jan 30.

25. Kao K.R. and Elinson R.P.,Dev Biol., 1989;127;64-77

26. Legay C in “ Why so many forms of AChE”. Microscopy Research and technique (2000), 49:56-72.

27. Manjrekar J and Krishnan K.S: Thermal Gels: A procedure for determination of heat-inactivation temperature of enzymes.Analytical Biochemistry 1987,160,409-413

28. Massoulie J, Anselmet A, Bon S, Krejci E, Legay C, Morel N, Simon S in “ The polymorphism of acetylcholinesterase: post- translational processing, quaternary associations and localization”, Chemico – Biological Interactions 119-120 (1999) 29-42.

29. Massoulie J: The Origin of molecular diversity and functional anchoring of cholinesterases. Neurosignals 2002,11:130-143.

30. Micales A.J and Bonde R.M. in “Isozymes: Methods and Applications”, Molecular Methods in Plant Pathology, chapter 9 (1995).

31. Peretz B, Srivatsan M. in “Chronic stimulation increases acetylcholinesterase activity in old Aplysia.” Behav Brain Res. 1996 Oct; 80(1-2): 203-10.

32. Pesando D., Huitorel P., Dolcini V., Angelini C., Guidetti P., Falugi C. in “Biological targets of neurotoxic pesticides analysed by

alteration of developmental events in the Mediterranean sea urchin, Paracentrotus lividus”. Marine Environmental Research 55 (2003) 39–57.

33. Piomboni P, Baccetti B, Moretti E, Gambera L, Angelini C, Falugi C in “Localization of molecules related to cholinergic signaling in eggs and zygotes of the sea urchin, Paracentrotus lividus.” J Submicrosc Cytol Pathol. 2001 Jan-Apr;33(1-2):187-93.

34. Raineri M. in “Histochemical investigations of Rotifera Bdelloidea. I. Localization of cholinesterase activity.” Histochem J. 1984 Jun;16(6):601-16.

35. Ros-Moreno R.M., De Armas-Serra C., Gimenez-Pardo c and Rodriguez-Caabeiro F., in “Comparison of Cholinesterase Activities in the Excretion-Secretion Products of Trichinella pseudospiralis and Trichinella spiralis Muscle Larvae.” Parasite,2002,9,153-159

36. Salceda R., Sanchez G. and Leon-cazares M.J in “Characterization of Cholinesterase Activities in Primary Cultures of Retinal Pigment Epithelium.” Investigative Ophthalmology and visual science,Vol.33,No.5,April 1992.

37. Salceda R. and Martinez T.M. in “ Characterization of Acetylcholinesterase and Butyrylcholinesterase Activities in Retinal Chick Pigment Epithelium During Development.” Exp Eye Res.(1992) 54, 17-22.

38. Sanchez-Chavez G. and Salceda R. in “Acetyl- and Butyrylcholinesterase in Normal and Diabetic Rat.” Neurochemical Research, Vol.26,No.2,2001,pp.153-159.

39. Sanchez-Chavez G. and Salceda R. in “Acetyl- and Butyrylcholinesterase in Normal and Streptozotocin-Diabetic Rat

Retinal Pigment Epithelium.” Neurochemistry International 39 (2001) 209-215.

40. Sanchez-Chavez G. and Salceda R. in “Effect of Streptozotocin-induced Diabetes on Activites of Cholinesterases in the Rat Retina.” IUBMB Life.49:283-287.2000

41. Sanchez-Chavez G., Vidal C.J. and Salceda R. in “Acetyl- and Butyrylcholinesterase Activities in the Rat Retina and Retinal Pigment Epithelium.” Journal of Neuroscience Research 41:655-662 (1995).

42. Soreq H, Seidman S. in "Acetylcholinesterase--new roles for an old actor", · Nat Rev Neurosci 2001 Sep; 2(9):670.

43. Srivatsan M, Peretz B. in “Acetylcholinesterase promotes regeneration of neurites in cultured adult neurons of Aplysia.” Neuroscience. 1997 Apr; 77(3): 921-31.

44. Sukhdeo SC, Sukhdeo MV, Mettrick DF in “Histochemical localization of acetylcholinesterase in the cerebral ganglia of Fasciola hepatica, a parasitic flatworm.” J Parasitol. 1988 Dec;74(6):1023-32.

45. Sutherland D, McClellan JS, Milner D, Soong W, Axon N, Sanders M, Hester A, Kao YH, Poczatek T, Routt S, Pezzementi L. in “Two cholinesterase activities and genes are present in amphioxus.” J Exp Zool. 1997 Feb 15;277(3):213-29.

46. Sutherland D., McClellan J., Milner D., Soong W, Axon N., Sanders M., Hester A., Kao Y., Poczatek T., Routt S, and Pezzementi L in “Two Cholinesterase Activities and Genes Are Present in Amphioxus.”The journal of experimental zoology277:213–229 (1997)

47. Talesa V, Grauso M, Giovannini E, Rosi G, Toutant “Solubilization, molecular forms, purification and substrate specificity

of two acetylcholinesterases in the medicinal leech(Hirudo medicinalis).”Biochem J. 1995 Mar 15;306 ( Pt 3):687-92.

48. Talesa V, Romani R, Antognelli C, Giovannini E, Rosi G in “Soluble and membrane-bound acetylcholinesterases in Mytilus galloprovincialis (Pelecypoda: Filibranchia) from the northern Adriatic sea.” Chem Biol Interact. 2001 Apr 16;134(2):151-66.

49. Talesa V, Romani R, Antognelli C, Giovannini E, Rosi G. in “Different expressions of organophosphate-resistant AChEs in the bivalve mollusk Scapharca inaequivalvis living in three different habitats.” Environ Toxicol Chem. 2002 Jan; 21(1): 102-8.

50. Tarrao A.S., Carmona F.M.M., Lindstrom and Britto L.R.G in “Expression of cholinergic system molecules during development of the chick nervous system.”Developmental Brain Research124(2000) 81-92.

51. Tayebati S., Assouad D., Ricci A., and Amenta F. in “ Immunochemical and immunocytochemical characterization of cholinergic markers in human peripheral blood lymphocytes.” Journal of Neurochemistry 132 (2002) 147-155.

52. Wallace BG, Gillon JW. in “Characterization of AChE in individual neurons in the leech central nervous system.”J Neurosci.1982Aug; 2(8): 1108-18.

53. Wilson W.B., Mettler A.M. and Asmundson V.R. in “ AChE and Non-specific Esterases in Developing Avian Tissues: Distribution and Molecular Weights of Esterases in Normal and Dystrophic Embryos and Chicks.” The Journal of Experimental Zoology. Vol.172, No.1, September 1969.


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