Studies on association of arbuscular mycorrhizal fungi with gluconacetobacter diazotrophicus and its effect on improvement of sorghum bicolor (L.)

Murugan Meenakshisundaram , Karrupagnaniar Santhaguru Int J Cur Sci Res. (2011) Studies on association of arbuscular mycorrhizal fungi with gluconacetobacter diazotrophicus and its effect on improvement of sorghum bicolor (L.). [Journal (Paginated)]

Full text available as:

PDF (Studies on association of arbuscular mycorrhizal fungi with gluconacetobacter diazotrophicus and its effect on improvement of sorghum bicolor (L.)) - Published Version
Available under License Creative Commons Attribution No Derivatives.



Considerable attention has been paid on endophytic diazotrophs in recent times, because of its of ability to fix and transfer fixed nitrogen to the host plant. Arbuscular mycorrhizal (AM) fungi are ubiquitous and play a significant role in improving the growth of plants through better uptake of nutrients, especially phosphorus. Interaction between AM fungi and nitrogen fixing bacteria and its impact on the host plant has been studied in several instances. In the present study, an attempt has been made to know the combining ability of G.diazotrophicus with AM fungi on S.bicolor. Spores of ten species of AM fungi were isolated from the rhizosphere soils of S.bicolor from different localities of Madurai and Sivagangai districts of Tamil Nadu. G.diazotrophicus was isolated from stem tissues of sugarcane (Saccharrum officinarum L.) from Madurai districts. The AM fungi in association with G.diazotrophicus were evaluated on the basis of root colonization, fresh and drymatter yield, N, P, soluble sugars and photosynthetic pigments in leaves of S.bicolor. Fresh weight and dry weight was significantly higher in dual inoculated plants. The highest values were recorded with Glomus fasciculatum + G.diazotrophicus combination. AM fungal infection was significantly higher in dual inoculated plants. N concentration was significantly increased by G.diazotrophicus even more in association with the efficient fungal strains. Dual inoculated plants showed a significant increase in P, soluble sugars, photosynthetic pigments in leaves was observed in G.diazotrophicus + Glomus fasciculatum combination. Such morphological modification may enhance water and nutrient uptake. Our results confirm the importance of studying plant-microbial interrelationship to provide useful information for agricultural system management.

Item Type:Journal (Paginated)
Keywords:G.diazotrophicus, Arbuscular Mycorrhizae, Sorghum bicolor, Glomus fasciculatum, Nitrogen fixation
ID Code:7304
Deposited By: CurrentSciDirect Publications, International Journal of Current Scientific Research (IJCSR)
Deposited On:02 May 2011 15:53
Last Modified:02 May 2011 15:53

References in Article

Select the SEEK icon to attempt to find the referenced article. If it does not appear to be in cogprints you will be forwarded to the paracite service. Poorly formated references will probably not work.

[1] Bonciarelli F. 1989. Fondamenti di Agronomia Generale. Edegricole. Bologna. 1989; 2: 1-3.

[2] Raman N, Mahadevan A. Mycorrhizal research – a priority in Agriculture. In Concepts in Mycorrhizal Research [K.G. Mukerji (ed.)], 1996; 2: 41-75.

[3] Jeffries P. Use of mycorrhizae in agriculture. Crit.Rev.Biotech. 1987; 5: 319-348.

[4] Kennedy IR, Tchan YT. Biological nitrogen in non-leguminous field crops: Recent advances. Plant Soil. 1992; 141: 93-118.

[5] Cavalcante VA, Dobereiner J. A new acid-tolerant nitrogen fixing bacterium associated with sugarcane. Plant Soil. 1988; 108: 23-31.

[6] Jimnez-Salgado T, Fuentez-Ramirez LE, Tapia-Hernandez A, Miguel A, Esparza M, Maritinez-Romero E, Caballero-Mellado J. Coffea Arabica L., a new host plant for Acetobacter diazotrophicus and isolation of other nitrogen fixing Acetobacteria. Env.Microbiol. 1997; 63: 3676-3683.

[7] Loganathan P, Sunitha R, Parida AK, Nair S. Isolation and characterization of two genetically distant groups of Acetobacter diazotrophicus from a new host plant Eleusine corocana L. Appl. Microbiol. 1999; 87: 167-172.

[8] Hernandez AT, Bustillos-Cristales MR, Jimnez-Salgado T, Cabellero-Mellado J, Fuentez-Ramirez LE. Natural endophytic occurrence of Acetobacter diazotrophicus in pineapple plants. Microb.Ecol. 2000; 39: 49-55.

[9] Loganathan P, Nair S. Crop-specific endophytic colonization by a novel, salt-tolerant, Nitrogen fixing and phosphate –solubilizing Gluconacetobacter sp. From wild rice. Biotechnol.Lett. 2003; 25, 497-501.

[10] Teixeira KRS, Stephan MP, Dobereiner J. Physiological studies of Saccharobacter nitrocaptans a new acid tolerant nitrogen fixing bacteria.4th international symposium on nitrogen fixation with non legumes, Rio de, Janeiro, Final program abstract, 1987; p-149.

[11] Bagyaraj DJ, Menge JA. Interaction between a VAM and Acetobacter and their effects on rhizosphere microflora and plant growth. New phytol. 1978; 80: 567-573.

[12] Boddey RM, Urquiaga S., Reis V, Dobereiner J. Biological nitrogen fixation associated with sugar cane. Plant and Soil. 1991; 137: 111-117.

[13] Dhillion S. Dual inoculation of pretransplant stage Oryza sativa L. plants with indigenous vesicular-arbuscular mycorrhizal fungi and fluorescent Psudomonas spp. Biol.Fertil. Soils. 1992; 13: 147-151.

[14] Paula MA, Reis VM, Dobereiner J. Interactions of Glomus clarum with Acetobacter diazotrophicus in infection of sweet potato (Ipomoea batatas), sugarcane (Saccharum spp), and sweet sorghum (Sorghum vulgare). Biol. Fertil.Soils. 1991; 11: 111-115.

[15] Paula MA, Urquiaga S, Siqueira JO, Dobereiner J. Synergistic effects of vesicular-arbuscular mycorrhizal fungi and diazotrophic bacteria on nutrition and growth of sweet potato (Ipomea batatas). Biol.Fertil.Soils. 1992; 14: 61-66.

[16] Jackson ML. Soil chemical analysis. Prentice Hall, New Delhi. 1971; 2: 1-5.

[17] Muthukumar T, Udaiyan K, Manian S. Vesicular-arbuscular mycorrhiza in tropical sedges of southern India. Biol.Fertil.Soils. 1996; 22: 96-100.

[18] Schenck NC, Perez Y. Manual for the identification of VA mycorrhizal fungi, 2nd ed. INVAM. University of Florida, Gianesville, 1990. 2: 241.

[19] Koske RE, Gemma JN. A modified procedure for staining roots to detect VA mycorrhizas. Mycological Research. 1989; 92: 488-505.

[20] McGonigle TP, Miller MH, Evans DG, Fairchid GL, Swan JA. A method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol. 1990; 115: 495-501.

[21] Hoagland DR, Arnon DI. The water-culture method for growing plants without soil. California Agricultural Experimental station circular University of Californi, 1950; 347: 12-15.

[22] Bergersen FJ. Measurement of nitrogen fixation by direct means. In: Methods for evaluating nitrogen fixation. F.J. Bergersen, ed. Wiley, Chichester, New York. 1980; 3: 65-110.

[23] Bartlett GR. Phosphorus assay in column chromatography. J. Biol.Chem. 1959; 234: 446-468.

[24] Mooris DL. Quantitative determination of carbohydrate with dry wood anthrone reagent. Science. 1948; 107: 254-255.

[25] Arnon DI. 1949. Copper enzymes in isolated chloroplasts polyphenol oxidases in Beta vulgaris. Plant physiology. 24: 1-15.

[26] Duncan DB. Multiple range and multiple f-tests. Biometrics. 1955; 11: 1-42.

[27] James EK,Olivares FL. Infection of sugarcane and other graminaceous plants by endophytic diazotrophs. Crit. Rev. In. Plant.Sci. 1997; 17: 77-119.

[28] Johnsen NC, Copealed PI, Crookston RK, Pfleger FL. Mycorrhizae: a possible explanation for yield decline associated with continues cropping. Agron, J. 1992; 84: 387-390.

[29] Abbot LK, Robson R. Growth of subterranean clover in relation to the formation of endomycorrhizas by introduced and indigenous fungi in a field soil. New Phytol . 1978; 81: 575-587.

[30] Harley JL, Smith SE. Mycorrhizal symbiosis. Academic press, New York. 1983.

[31] Sieverding E. Vesicular-arbuscular mycorrhiza management in tropical agro ecosystems. Technical co operation, Federal Rebublic of Germany, 1991; 8805: 462-465.

[32] Ruiz-Lozano JM, Azcon R. Hyphal contribution to water uptake in mycorrhizal plants as affected by the fungal species and water stress. Physiologia Plantarum. 1995; 95: 472-478.

[33] Giovannetti M, Sbrana C. Meeting a non-host: behavior of AM fungi. Mycorrhiza. 1998; 8: 123-130.

[34] Vinayak K, Bagyaraj DJ. Selection of efficient VA mycorrhizal fungi for Trifoliate organe. Biol.Agric.Hortic. 1990; 6: 305-311.

[35] Giovanetti M. Some anatomic feautures and spore germination of the vesicular-arbuscular endophyte Glomus clarum Nicolson and Schenck. Annali di Microbiologia. 1981; 31: 103-107.

[36] Isopi R, Fabbri P, Delgallo M, Puppi G. Dual inoculation of Sorghum bicolor (L.) Monech ssp with vesicular arbuscular mycorrhizas and Acetobacter diazotrophicus. Symbiosis. 1995; 18: 43-55.

[37] Fuentes-Ramirez LE, Jimnez-Salgado T, Abarca-Ocampo IR, Caballero-Mellado J. Acetobacter diazotrophicus an indole acetic acid producing bacterium isolated from sugarcane cultivars of Mexico. Plant Soil. 1993; 154: 145-150.

[38] Okon Y, Labandera-Gonzalez CA. Agronomic applications of Azospirillum and evaluation of twenty years world wide field inoulation. Soil.Biol. Biochem. 1994; 26: 1591-1601.

[39] Lee S, Reth A, Meletzus D, Sevilla M, Kennedy C. Characterization of a major cluster of nif, fix and associated genes in a sugarcane endophyte Acetobacter diazotrophicus. J.Bacteriol. 2000; 182: 7088-7091.

[40] Kumarasamy V. Studies on diazotrophs associated with grasses and their rhizosphere in southern Tamil Nadu. Ph.D., thesis, Dept.of. Botany, Thiagarajar College, Madurai-9, Tamil Nadu, India. 2002.

[41] Sevilla M, Kennedy C. Genetic analysis of nitrogen fixation and plant-growth stimulating properties of Acetobacter diazotrophicus, an endophyte of sugarcane. In: Nitrogen fixation in bacteria: Molecular cell biology. 1998; 14: 564-569.

[42] Manjunath A, Bagyaraj DJ, Gowda HSG. Dual inoculation with VA mycorrhiza and Rhizobium is beneficial to Leucaena. Plant Soil. 1984; 78: 445-448.

[43] Pocovsky RS, Fuller G, Stafford AE. Nutrient and growth interactions in soybean colonized with Glomus fasciculatum and Rhizobium japonicum. Plant Soil. 1986; 92: 37-45.

[44] Azcon R, Rubia R, Barea JM. Selective interactions between different speices of mycorrhizal fungi and Rhizobium meliloti strains and their effects on growth, nitrogen fixation and nutrition of Medicago sativa. New phytol. 1991; 117: 399-404.

[45] Xavier LJC, Germida A. Response of lentil to co-inoculation with arbuscular mycorrhizal fungi and rhizobia varying in efficacy. Soil.Biol.Biochem. 2002; 34: 181-188.

[46] Tester M, Smith SE, Smith FG, Walker NA. Effect of photon irradiance on the growth of shoots and roots, on the rate of intiation of mycorrhizal infection and on the growth of infections units in Trifolium subterraneum (L). New Phytol. 1986; 103: 375-390.

[47] Sivaprasad P, Rai PV. Mechanisms of enhanced nodulation in vesicular arbuscular mycorrhizal in Pigeon pea, and Cajanus cajan (L). Agric.Rus.J. 1987; 25: 99-102.


Repository Staff Only: item control page