creators_name: Soogarun, Suphan
creators_name:  Choewbamrungkiat, Montri
creators_name:  Wiwanitkit, Viroj
creators_name: Suwansaksri, Jamsai
creators_name: Nopanitaya, Waykin
creators_name: Pradniwat, Paweena
creators_name: Palasuwan, Attakorn
creators_name: Chanprasert, Supantitra
creators_id: Soogarun S
creators_id: Choewbamrungkiat M
creators_id: Wiwanitkit V
creators_id: Suwansaksri J
creators_id: Nopanitaya W
creators_id: Pradniwat P
creators_id: Palasuwan A
creators_id: Chanprasert S
editors_name: Kakkilaya, Srinivas
editors_id: Kakkilaya BS
type: journale
datestamp: 2006-12-22
lastmod: 2011-03-11 08:56:45
metadata_visibility: show
title: Study on the effect of green pit viper venom (Trimeresurus albolabris) on platelet morphology by flow cytometry
ispublished: pub
subjects: OJHAS
full_text_status: public
keywords: Green pit viper, Venom, Platelet
abstract: The incidence of venomous snake bites, especially by green pit viper, has been increasing every year in Thailand. The bite of green pit viper causes bleeding because of thrombin–like property of the venom and it has been reported that the mean platelet volume decreases in those bitten by this snake. The objective of this study was to study the effect of green pit viper venom (Trimeresurus albolabris) on platelet volume (MPV), number of platelets and platelet morphology in vitro. The test was carried out by washing platelet in phosphate buffer pH 7.2 so as to get rid of fibrinogen, then the washed platelets were mixed with green pit viper venom. The mean platelet volume and number of platelets were determined by flow cytometry. The results showed that there was a decrease in the number of platelets (216±101 x 109/l and 78.1±43.4 x 109/l; P< 0.05) and also the MPV (8.9±1.2fl and 4.8±1.3fl, P< 0.05). The  platelet size was smaller than normal, ranging from 1.1-1.2 micrometers. In conclusion, the green pit viper venom had a direct effect on platelet morphology, especially by decreasing platelet volume and numbers.
date: 2006-12
date_type: published
publication: Online Journal Of Health And Allied Sciences
volume: 5
number: 3
publisher: Dr. B.S. Kakkilaya
refereed: TRUE
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citation:   Soogarun, Suphan and  Choewbamrungkiat, Montri and  Wiwanitkit, Viroj and Suwansaksri, Jamsai and Nopanitaya, Waykin and Pradniwat, Paweena and Palasuwan, Attakorn and Chanprasert, Supantitra  (2006) Study on the effect of green pit viper venom (Trimeresurus albolabris) on platelet morphology by flow cytometry.  [Journal (On-line/Unpaginated)]     
document_url: http://cogprints.org/5327/1/2006-3-5.pdf