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--- abstract: 'The incidence of venomous snake bites, especially by green pit viper, has been increasing every year in Thailand. The bite of green pit viper causes bleeding because of thrombin–like property of the venom and it has been reported that the mean platelet volume decreases in those bitten by this snake. The objective of this study was to study the effect of green pit viper venom (Trimeresurus albolabris) on platelet volume (MPV), number of platelets and platelet morphology in vitro. The test was carried out by washing platelet in phosphate buffer pH 7.2 so as to get rid of fibrinogen, then the washed platelets were mixed with green pit viper venom. The mean platelet volume and number of platelets were determined by flow cytometry. The results showed that there was a decrease in the number of platelets (216±101 x 109/l and 78.1±43.4 x 109/l; P< 0.05) and also the MPV (8.9±1.2fl and 4.8±1.3fl, P< 0.05). The platelet size was smaller than normal, ranging from 1.1-1.2 micrometers. In conclusion, the green pit viper venom had a direct effect on platelet morphology, especially by decreasing platelet volume and numbers.' altloc: - http://www.ojhas.org/issue19/2006-3-5.htm chapter: ~ commentary: ~ commref: ~ confdates: ~ conference: ~ confloc: ~ contact_email: ~ creators_id: - Soogarun S - Choewbamrungkiat M - Wiwanitkit V - Suwansaksri J - Nopanitaya W - Pradniwat P - Palasuwan A - Chanprasert S creators_name: - family: Soogarun given: Suphan honourific: '' lineage: '' - family: ' Choewbamrungkiat' given: Montri honourific: '' lineage: '' - family: ' Wiwanitkit' given: Viroj honourific: '' lineage: '' - family: Suwansaksri given: Jamsai honourific: '' lineage: '' - family: Nopanitaya given: Waykin honourific: '' lineage: '' - family: Pradniwat given: Paweena honourific: '' lineage: '' - family: Palasuwan given: Attakorn honourific: '' lineage: '' - family: Chanprasert given: Supantitra honourific: '' lineage: '' date: 2006-12 date_type: published datestamp: 2006-12-22 department: ~ dir: disk0/00/00/53/27 edit_lock_since: ~ edit_lock_until: ~ edit_lock_user: ~ editors_id: - Kakkilaya BS editors_name: - family: Kakkilaya given: Srinivas honourific: Dr. lineage: Bevinje eprint_status: archive eprintid: 5327 fileinfo: /style/images/fileicons/application_pdf.png;/5327/1/2006%2D3%2D5.pdf full_text_status: public importid: ~ institution: ~ isbn: ~ ispublished: pub issn: ~ item_issues_comment: [] item_issues_count: 0 item_issues_description: [] item_issues_id: [] item_issues_reported_by: [] item_issues_resolved_by: [] item_issues_status: [] item_issues_timestamp: [] item_issues_type: [] keywords: 'Green pit viper, Venom, Platelet' lastmod: 2011-03-11 08:56:45 latitude: ~ longitude: ~ metadata_visibility: show note: ~ number: 3 pagerange: ~ pubdom: TRUE publication: Online Journal Of Health And Allied Sciences publisher: Dr. B.S. Kakkilaya refereed: TRUE referencetext: |- 1. Dumavibhat B. A study of epimiology, risk factors and preventive measures against snake bites. J Med Assoc Thai 1997;80(9):547-556 2. Visudhiphan S, Dumavibhat B, Trishnananda M. Prolonged defibrination syndrome after green pit viper bite with persisting venom activity in patient’s blood. Am J Clin Pathol 1981;75(1):65-69 3. Kamnerdnond C, Jitprommeta J. Using green pit viper venom for coagulation study. A thesis , Faculty of Allied Health Sciences, Chulalongkorn University, 2004 4. Rojnuckarin P, Intragumtornchai T, Sattapiboon R, Muanpasitporn C, Pakmanee N, Khow O, Swasdikul D. The effects of green pit viper (Trimeresurus albolabris and Trimeresurus macrops) venom on the fibrinolytic system in human. Toxicon. 1999;37(5):743-755 5. Soogarun S, Wiwanitkit V, Suwansaksri J. A trend of platelet indices in patients with green pit viper toxin. Clin Appl Thromb Hemost. 2003;9(4):337-339 6. Soogarun S, Chiowbamrungkiat, Lertlum T, Pradniwat P, Jarujaron S, Palasuwan A, Jitprommetta, Kamnerdnond C. Does green pit viper (Trimeresurus albolabris) venom act against antithrombin III? Hema 2005;8(3):532-533 7. Common buffer (Online, Cited 2005 Sep 30). Available at: http:/www.ou.edu/research/electron/bmz5364/buffers.html 8. Wiwanitkit V, Suwansaksri J. Effect of green pit viper toxin on red blood cell index (an interim analysis). Toxicology 2001;164 (suppl):178 9. Nopathorn S, Tejachokviwat M, Maneesri S, Kasantikul V. Effects of Russell’s viper venom on human erythrocytes in vitro. J Nat Toxins. 1998;7(1):73-85 relation_type: [] relation_uri: [] reportno: ~ rev_number: 12 series: ~ source: ~ status_changed: 2007-09-12 17:09:08 subjects: - OJHAS succeeds: ~ suggestions: ~ sword_depositor: ~ sword_slug: ~ thesistype: ~ title: Study on the effect of green pit viper venom (Trimeresurus albolabris) on platelet morphology by flow cytometry type: journale userid: 4338 volume: 5