?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=Regional+distribution+of+the+prostaglandin+E2+receptor+EP1+in+the+rat+brain%3A+accumulation+in+Purkinje+cells+of+the+cerebellum&rft.creator=Candelario-Jalil%2C+Eduardo&rft.creator=Slawik%2C+Helen&rft.creator=Ridelis%2C+Ingrid&rft.creator=Waschbisch%2C+Anne&rft.creator=Akundi%2C+Ravi+S.&rft.creator=Hull%2C+Michael&rft.creator=Fiebich%2C+Bernd+L.&rft.subject=Neurochemistry&rft.description=Prostaglandin+E2+(PGE2)%2C+is+a+major+prostanoid+produced+by+the+activity+of+cyclooxygenases+(COX)+in+response+to+various+physiological+and+pathological+stimuli.+PGE2+exerts+its+effects+by+activating+four+specific+E-type+prostanoid+receptors+(EP1%2C+EP2%2C+EP3+and+EP4).+In+the+present+study%2C+we+analyzed+the+expression+of+the+PGE2+receptor+EP1+(mRNA+and+protein)+in+different+regions+of+the+adult+rat+brain+(hippocampus%2C+hypothalamus%2C+striatum%2C+prefrontal+cerebral+cortex%2C+parietal+cortex%2C+brain+stem+and+cerebellum)+using+reverse+transcription-polymerase+chain+reaction+(RT-PCR)%2C+Western+blotting+and+immunohistochemical+methods.+On+a+regional+basis%2C+levels+of+EP1+mRNA+were+the+highest+in+parietal+cortex+and+cerebellum.+At+the+protein+level%2C+we+found+a+very+strong+expression+of+EP1+in+cerebellum+as+revealed+by+Western+blotting+experiments.+Furthermore%2C+the+present+study+provides+for+the+first+time+evidence+that+the+EP1+receptor+is+highly+expressed+in+the+cerebellum%2C+where+the+Purkinje+cells+displayed+a+very+high+immunolabeling+of+their+perikaryon+and+dendrites+as+observed+in+the+immunohistochemical+analysis.+Results+from+the+present+study+indicate+that+the+EP1+prostanoid+receptor+is+expressed+in+specific+neuronal+populations%2C+which+possibly+determine+the+region+specific+response+to+PGE2.&rft.publisher=Humana+Press&rft.contributor=Gozes%2C+Illana&rft.date=2005&rft.type=Journal+(Paginated)&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.identifier=http%3A%2F%2Fcogprints.org%2F5618%2F1%2FEP1_paper_J_Mol_Neurosci_2005.pdf&rft.identifier=++Candelario-Jalil%2C+Eduardo+and+Slawik%2C+Helen+and+Ridelis%2C+Ingrid+and+Waschbisch%2C+Anne+and+Akundi%2C+Ravi+S.+and+Hull%2C+Michael+and+Fiebich%2C+Bernd+L.++(2005)+Regional+distribution+of+the+prostaglandin+E2+receptor+EP1+in+the+rat+brain%3A+accumulation+in+Purkinje+cells+of+the+cerebellum.++%5BJournal+(Paginated)%5D+++++&rft.relation=http%3A%2F%2Fcogprints.org%2F5618%2F