--- abstract: 'Kainate induces a marked expression of cyclooxygenase-2 after its systemic administration. Because cyclooxygenase-2 activity is associated to the production of reactive oxygen species, we investigated the effects of nimesulide, a selective cyclooxygenase-2 inhibitor, on kainate-induced in vivo oxidative damage in the rat hippocampus. A clinically relevant dose of nimesulide (6 mg/kg, i.p. ) was administered three times following kainate application (9 mg/kg, i.p.). After 24 h of kainate administration, the drastic decrease in hippocampal glutathione content and the significant increase in lipid peroxidation were attenuated in nimesulide-treated rats, suggesting that the induction of cyclooxygenase-2 is involved in kainate-mediated free radicals formation.' altloc: - http://candelariojalil.googlepages.com/Eur_J_Pharmacol_Candelario-Jalil_et_al_2000.pdf chapter: ~ commentary: ~ commref: ~ confdates: ~ conference: ~ confloc: ~ contact_email: ~ creators_id: [] creators_name: - family: Candelario-Jalil given: Eduardo honourific: '' lineage: '' - family: Ajamieh given: Hussam honourific: '' lineage: '' - family: Sam given: Susana honourific: '' lineage: '' - family: Martinez given: Gregorio honourific: '' lineage: '' - family: Leon given: Olga S. honourific: '' lineage: '' date: 2000-03 date_type: published datestamp: 2007-08-20 department: ~ dir: disk0/00/00/56/53 edit_lock_since: ~ edit_lock_until: ~ edit_lock_user: ~ editors_id: [] editors_name: [] eprint_status: archive eprintid: 5653 fileinfo: /style/images/fileicons/application_pdf.png;/5653/1/Kainate_nimesulide_Eur_J_Pharmacol_2000.pdf full_text_status: public importid: ~ institution: ~ isbn: ~ ispublished: pub issn: ~ item_issues_comment: [] item_issues_count: 0 item_issues_description: [] item_issues_id: [] item_issues_reported_by: [] item_issues_resolved_by: [] item_issues_status: [] item_issues_timestamp: [] item_issues_type: [] keywords: kainate; excitotoxicity; cyclooxygenase-2; nimesulide; oxidative stress; hippocampus lastmod: 2011-03-11 08:56:56 latitude: ~ longitude: ~ metadata_visibility: show note: ~ number: 3 pagerange: 295-298 pubdom: FALSE publication: European Journal of Pharmacology publisher: Elsevier refereed: TRUE referencetext: | Adams, J., Collaço-Moraes, Y., de Belleroche, J., 1996. Cyclooxygenase-2 induction in cerebral cortex: an intracellular response to synaptic excitation. J. Neurochem. 66, 6-13. Baran, H., Heldt, R., Hertting, G., 1987. Increased prostaglandin formation in rat brain following systemic application of kainic acid. Brain Res. 404, 107-112. Baran, H., Vass, K., Lassmann, H., Hornykiewicz, O., 1994. The cyclooxygenase and lipoxygenase inhibitor BW755C protects rats against kainic acid-induced seizures and neurotoxicity. Brain Res. 646, 201-206. Ben Ari, Y., 1985. Limbic seizure and brain damage produced by kainic acid: mechanisms and relevance to human temporal lobe epilepsy. Neuroscience 14, 375-403. Breder, C. D., Dewitt, D., Kraig, R. P., 1995. Characterization of inducible cyclooxygenase in rat brain. J. Comp. Neurol. 355, 296-315. Carriedo, S. G., Yin, H. Z., Sensi, S. L., Weiss, J. H., 1998. Rapid Ca2+ entry through Ca2+-permeable AMPA/kainate channels triggers marked intracellular Ca2+ rises and consequent oxygen radical production. J. Neurosci. 18, 7727-7738. Cheng, Y., Sun, A. Y., 1994. Oxidative mechanisms involved in kainate-induced cytotoxicity in cortical neurons. Neurochem. Res. 19, 1557-1564. Choi, D. W., Rothman, S. M., 1990. The role of glutamate neurotoxicity in hypoxic ischaemic neuronal death. Annu. Rev. Neurosci. 13, 171-182. Dykens, J. A., Stern, A., Trenkner, E., 1987. Mechanisms of kainate toxicity to cerebellar neurons in vitro is analogous to reperfusion tissue injury. J. Neurochem. 49, 1222-1228. Ellman, G. J., 1959. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82, 70-77. Ferkany, J. W., Zaczek, R., Coyle, J. T., 1982. Kainic acid stimulates excitatory amino acid neurotransmitter release at presynaptic receptors. Nature 298, 757-759. Hashimoto, K., Watanabe, K., Nishimura, T., Iyo, M., Shirayama, Y., Minabe, Y., 1998. Behavioral changes and expression of heat shock protein hsp-70 mRNA, brain-derived neurotrophic factor mRNA, and cyclooxygenase-2 mRNA in rat brain following seizures induced by systemic administration of kainic acid. Brain Res. 804, 212-223. Heggli, D. E., Malthe-Sörenssen, D., 1982. Systemic injection of kainic acid: effect on neurotransmitter markers in piriform cortex, amygdaloid complex and hippocampus and protection by cortical lesioning and anti-convulsants. Neuroscience 7, 1257-1264. Nakayama, M., Uchimura, K., Zhu, R. L., Nagayama, T., Rose, M. E., Stetler, R. A., Isakson, P. C., Chen, J., Graham, S. H., 1998. Cyclooxygenase-2 inhibition prevents delayed death of CA1 hippocampal neurons following global ischemia. Proc. Natl. Acad. Sci. USA 95, 10954-10959. Nogawa, S., Zhang, F., Ross, M. E., Iadecola, C., 1997. Cyclo-Oxygenase-2 gene expression in neurons contributes to ischemic brain damage. J. Neurosci. 17, 2746-2755. Planas, A. M., Soriano, M. A., Rodríguez-Farré, E., Ferrer, I., 1995. Induction of cyclooxygenase-2 mRNA and protein following transient focal ischemia in the rat brain. Neurosci. Lett. 200, 187-190. Sandhya, T. L., Ong, W. Y., Horrocks, L. A., Farooqui, A. A., 1998. A light and electron microscopic study of cytoplasmic phospholipase A2 and cyclooxygenase-2 in the rat hippocampus after kainate lesions. Brain Res. 788, 223-231. Sanz, O., Estrada, A., Ferrer, I., Planas, A.M., 1997. Differential cellular distribution and dynamics of HSP70, cyclooxygenase-2, and c-FOS in the rat brain after transient focal ischemia or kainic acid. Neuroscience 80, 221-232. Seibert, K., Masferrer, J., Zhang, Y., Gregory, S., Olson, G., Hauser, S., Leahy, K., Perkins, W., Isakson, P., 1995. Mediation of inflammation by cyclooxygenase-2. Agents Actions Suppl. 46, 41-50. Spector, T., 1978. Refinement of the Coomassie Blue method of protein quantification. Anal. Biochem. 86, 142-146. Sperk, G., Lassmann, H., Baran, H., Seitelberger, F., Hornykiewicz, O., 1985. Kainic acid-induced seizures: dose-relationship of behavioral, neurochemical and histopathological changes. Brain Res. 338, 289-295. relation_type: [] relation_uri: [] reportno: ~ rev_number: 12 series: ~ source: ~ status_changed: 2007-09-12 17:11:16 subjects: - neuro-chem succeeds: ~ suggestions: ~ sword_depositor: ~ sword_slug: ~ thesistype: ~ title: Nimesulide limits kainate-induced oxidative damage in the rat hippocampus type: journalp userid: 6903 volume: 390