--- abstract: This information is provided on Cogprints for colleagues in the Fragile X field who have requested it directly in the past. It is also a companion work to the article “Human Fragile X gene locus P1 artificial chromosome transgenic mice” from our group (manuscript to be made available on Cogprints). altloc: [] chapter: ~ commentary: ~ commref: ~ confdates: ~ conference: ~ confloc: ~ contact_email: ~ creators_id: - rpb3@columbia.edu creators_name: - family: Bauchwitz given: Robert honourific: 'Dr. ' lineage: '' date: 2009-12-04 date_type: published datestamp: 2009-12-19 12:22:18 department: ~ dir: disk0/00/00/67/38 edit_lock_since: ~ edit_lock_until: 0 edit_lock_user: ~ editors_id: [] editors_name: [] eprint_status: archive eprintid: 6738 fileinfo: application/pdf;http://cogprints.org/6738/1/FX_Ab_Bauchwitz2009.pdf full_text_status: public importid: ~ institution: ~ isbn: ~ ispublished: pub issn: ~ item_issues_comment: [] item_issues_count: 0 item_issues_description: [] item_issues_id: [] item_issues_reported_by: [] item_issues_resolved_by: [] item_issues_status: [] item_issues_timestamp: [] item_issues_type: [] keywords: 'Fragile X syndrome, antibodies, fmr1-tm1Cgr, mouse model, protein expression ' lastmod: 2011-09-17 17:47:07 latitude: ~ longitude: ~ metadata_visibility: show note: "The information presented here is summarized from the following sources: \r\n\r\n1) Analysis of FMR1 PAC transgenic and fmr1tm1cgr knockout mice \r\nRobert Bauchwitz M.D., Ph.D.\r\nThe Banbury Center, Cold Spring Harbor Laboratory (2000) \r\n(Invited Lecture) \r\n\r\n2) FMR1 Transgenesis: Animal Models and Therapeutic Considerations\r\nRobert Bauchwitz M.D., Ph.D.\r\nProceedings of the 7th International Fragile X Foundation Conference, National Fragile X Foundation, (2000) Sec.3\r\n(Invited Lecture and Published Summary) \r\n\r\n3) The fmr1tm1cgr knockout mouse is not a molecular null: implications for phenotype \r\nRobert Bauchwitz M.D., Ph.D.\r\nThe Banbury Center, Cold Spring Harbor Laboratory (2001) \r\n(Invited Lecture) \r\n" number: ~ pagerange: ~ pubdom: TRUE publication: ~ publisher: ~ refereed: FALSE referencetext: "Bauchwitz, R., Costantini, F., 1998. YAC transgenesis: a study of conditions to protect YAC DNA from breakage and a protocol for transfection. Biochimica et Biophysica Acta 1401, 21-37. \r\n\r\nDevys, D., Lutz, Y., Rouyer, N., Bellocq, J. P., Mandel, J. L., 1993. The FMR-1 protein is cytoplasmic, most abundant in neurons and appears normal in carriers of a fragile X premutation. Nature Genetics 4, 335-340. \r\n\r\nKhandjian, E. W., Bardoni, B., Corbin, F., Sittler, A., Giroux, S., Heitz, D., Tremblay, S., Pinset, C., Montarras, D., Rousseau, F., Mandel, J., 1998. Novel isoforms of the fragile X related protein FXR1P are expressed during myogenesis. Human Molecular Genetics 7, 2121-2128. \r\n\r\nKhandjian, E. W., Fortin, A., Thibodeau, A., Tremblay, S., Cote, F., Devys, D., Mandel, J. L., Rousseau, F., 1995. A heterogeneous set of FMR1 proteins is widely distributed in mouse tissues and is modulated in cell culture. Human Molecular Genetics 4, 783-789. \r\n\r\nMarahrens, Y., Panning, B., Dausman, J., Strauss, W., Jaenisch, R., 1997. Xist-deficient mice are defective in dosage compensation but not spermatogenesis [see comments]. Genes and Development 11, 156-166. \r\n\r\nPeier, A. M., McIlwain, K. L., Kenneson, A., Warren, S. T., Paylor, R., Nelson, D. L., 2000. (Over)correction of FMR1 deficiency with YAC transgenics: behavioral and physical features. Human Molecular Genetics 9, 1145-1159. \r\n\r\nSittler, A., Devys, D., Weber, C., Mandel, J. L., 1996. Alternative splicing of exon 14 determines nuclear or cytoplasmic localisation of fmr1 protein isoforms. Human Molecular Genetics 5, 95-102. \r\n\r\nTamanini, F., Willemsen, R., van Unen, L., Bontekoe, C., Galjaard, H., Oostra, B. A., Hoogeveen, A. T., 1997. Differential expression of FMR1, FXR1 and FXR2 proteins in human brain and testis. Human Molecular Genetics 6, 1315-1322. \r\n\r\nVandeberg, J. L., 1983. Developmental aspects of X chromosome inactivation in eutherian and metatherian mammals. Journal of Experimental Zoology 228, 271-286. \r\n\r\nVerheij, C., de Graaff, E., Bakker, C. E., Willemsen, R., Willems, P. J., Meijer, N., Galjaard, H., Reuser, A. J., Oostra, B. A., Hoogeveen, A. T., 1995. Characterization of FMR1 proteins isolated from different tissues. Human Molecular Genetics 4, 895-901. \r\n\r\nYan, Q. J., Asafo-Adjei, P. K., Arnold, H. M., Brown, R. E., Bauchwitz, R. P., 2004. A phenotypic and molecular characterization of the fmr1-tm1Cgr Fragile X mouse. Genes, Brain and Behavior 3, 337-359. \r\n\r\nZhang, Y., O'Connor, J. P., Siomi, M. C., Srinivasan, S., Dutra, A., Nussbaum, R. L., Dreyfuss, G., 1995. The fragile X mental retardation syndrome protein interacts with novel homologs FXR1 and FXR2. EMBO Journal 14, 5358-5366.\r\n" relation_type: [] relation_uri: [] reportno: ~ rev_number: 22 series: ~ source: ~ status_changed: 2009-12-19 12:22:18 subjects: - neurgen succeeds: ~ suggestions: ~ sword_depositor: ~ sword_slug: ~ thesistype: ~ title: 'Characterization of Fragile X mental retardation antibodies for use in cross-species immunoblotting, immunohistochemistry, and electron microscopy' type: other userid: 4550 volume: ~