?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=A+comparison+between+the+effects+of+three+potential+scar-reducing+agents+applied+at+a+site+of+sciatic+nerve+repair&rft.creator=Ngeow%2C+Dr.+W.C.&rft.creator=Atkins%2C+S.&rft.creator=Morgan%2C+C.+R.&rft.creator=Metcalfe%2C+A.+D.&rft.creator=Boissonade%2C+F.+M.&rft.creator=Loescher%2C+A.+R.&rft.creator=Robinson%2C+P.+P.&rft.subject=JOURNALS&rft.subject=Online+Journal+of+Health+and+Allied+Sciences&rft.description=We+have+investigated+the+effect+of+three+potential+scar-reducing+agents+applied+at+a+sciatic+nerve+repair+site+in+C57-black-6+mice.+Under+anaesthesia+the+nerve+was+transected%2C+repaired+using+four+epineurial+sutures%2C+and+100+mu+l+of+either+triamcinolone+acetonide+(1+mg%2F100+mu+l)%2C+an+interleukin-10+peptide+fragment%3A+(125+ng%2F100+mu+l+or+500+ng%2F100+mu+l)+or+mannose-6-phosphate+(M6P%2C+200+mM+or+600+mM)+was+injected+into+and+around+the+nerve.+After+6+weeks+the+extent+of+regeneration+was+assessed+electrophysiologically+by+determining+the+ratio+of+the+compound+action+potential+(CAP)+modulus+evoked+by+electrical+stimulation+of+the+nerve+2+mm+distal+or+proximal+to+the+repair+site.+The+conduction+velocity+of+the+fastest+components+in+the+CAP+was+also+calculated.+The+percentage+area+of+collagen+staining+(PAS)+at+the+repair+site+was+analysed+using+Picrosirius+Red+and+image+analysis.+Comparisons+were+made+with+a+placebo+group+(100+mu+l+of+phosphate+buffered+saline)+and+sham-operated+controls.+The+median+CAP+modulus+ratio+in+the+600+mM+M6P+group+was+0.44%2C+which+was+significantly+higher+than+in+the+placebo+group+(0.24%2C+P%3D0.012%3A+Kruskal-Wallis+test).+Conduction+velocities+were+also+faster+in+the+600+mM+M6P+group+(median+30+m+s(-1))+than+in+the+placebo+group+(median+27.8+m+s(-1)%3B+P%3D0.0197%3A+Kruskal-Wallis+test).+None+of+the+other+treated+groups+were+significantly+different+from+the+placebo%2C+and+all+had+significantly+lower+CAP+ratios+than+the+sham+controls+(P%7B%5Censuremath%7B%3C%7D%7D0.05).+All+repair+groups+had+a+significantly+higher+PAS+for+collagen+than+sham+controls.+We+conclude+that+the+administration+of+600+mM+mannose-6-phosphate+to+a+nerve+repair+site+enhances%3B+axonal+regeneration.+(C)+2011+IBRO.+Published+by+Elsevier+Ltd.+All+rights+reserved.&rft.publisher=Publisher%3A+PERGAMON-ELSEVIER+SCIENCE+LTD%2C+THE+BOULEVARD%2C+LANGFORD+LANE%2C+KIDLINGTON%2C+OXFORD+OX5+1GB%2C+ENGLAND&rft.date=2011-05&rft.type=Journal+(Paginated)&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.identifier=http%3A%2F%2Fcogprints.org%2F8169%2F1%2FA%2520COMPARISON%2520BETWEEN%2520THE%2520EFFECTS%2520OF%2520THREE%2520POTENTIAL%2520SCAR.pdf&rft.identifier=++Ngeow%2C+Dr.+W.C.+and+Atkins%2C+S.+and+Morgan%2C+C.+R.+and+Metcalfe%2C+A.+D.+and+Boissonade%2C+F.+M.+and+Loescher%2C+A.+R.+and+Robinson%2C+P.+P.++(2011)+A+comparison+between+the+effects+of+three+potential+scar-reducing+agents+applied+at+a+site+of+sciatic+nerve+repair.++%5BJournal+(Paginated)%5D+++++&rft.relation=http%3A%2F%2Fcogprints.org%2F8169%2F