Emerging quinolones resistant transfer genes among gram-negative bacteria, isolated from faeces of HIV/AIDS patients attending some Clinics and Hospitals in the City of Benin, Edo State, Nigeria

I O, Enabulele and S C, Yah and E O, Yusuf and N O, Eghafona (2006) Emerging quinolones resistant transfer genes among gram-negative bacteria, isolated from faeces of HIV/AIDS patients attending some Clinics and Hospitals in the City of Benin, Edo State, Nigeria. [Journal (On-line/Unpaginated)]

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A survey of 1431 gram-negative bacilli from June 2001 to September 2005 were obtained from the faeces of 920 HIV/AIDS patients attending some Clinics and Hospitals in Benin City, Nigeria, were screened for quinolones resistance gene. The HIV/AIDS patients CD4 cells range was ≤14/mm3 ≥800/mm3 of blood. Out of the 1431 isolates, 343 (23.9%) were resistance to quinolones with a MIC ≥4μg/ml for norfloxacin, ciprofloxacin and pefloxacin while a MIC of ≥32 µg/ml for nalidixic acid. The screened isolates include Pseudomonas aeruginosa 64(18.7%), E coli 92(26.8%), Klebsiella pneumoniae 53(15.4%), Salmonella typhi 39(11.4%), Shigella dysenteriae 36(10.5%), Proteus mirabilis 34(9.9%) and Serratia marcescens 25(7.3%). The average resistance of the isolates to the various quinolones ranged from 42.7% to 66.7%. Klebsiella were the most resistant isolates with a mean resistance of 66.7% while Proteus were the less resistant isolates with a mean resistance of 42.7%. Most isolates were resistant to Nalidixic acid followed by norfloxacin while the less resistant were to the pefloxacin. The frequency of qnr genes transfer to EJRifr as recipient ranged from 2 x 10-2 to 6 x 10-6 with an average of 2 plasmids per cell. The molecular weight of the plasmids ranged from <2.9kbp to <5.5 kbp. This indicated that plasmids allowed the movement of genetic materials including qnr resistant genes between bacteria species and genera in Benin City, Nigeria

Item Type:Journal (On-line/Unpaginated)
Keywords:Resistance Gene Transfer, HIV/AIDS, Gram-negative bacilli
Subjects:JOURNALS > Online Journal of Health and Allied Sciences
ID Code:5322
Deposited By: Kakkilaya Bevinje, Dr. Srinivas
Deposited On:22 Dec 2006
Last Modified:11 Mar 2011 08:56

References in Article

Select the SEEK icon to attempt to find the referenced article. If it does not appear to be in cogprints you will be forwarded to the paracite service. Poorly formated references will probably not work.

1. World Health Organization (WHO). Deaths by cause, sex and mortality stratum in WHO Regions, estimates for 2001. World Health Report. 2002. Geneva: WHO.

2. Gilligan PH. Therapeutic challenges posed by bacterial bioterrorism threats. Curr Opin Microbiol 2002;5:489-95

3. Smolinski MS, Hamburg MA, Lederberg J. Microbial threats to health: emergence, detection, and response. 2003. Washington, DC: Institute of Medicine.

4. Rukujei AD. Epidemiology of HIV/AIDS in Nigeria. Nig. J. of Med. 1998;7:8-9.

5. Akinsete I. Clinical management of HIV/AIDS. Nig. J. Med 1998;7:19.

6. Whitworths J, Morgan D, Mayonja. Effect of HIV-1 and increasing immuno-suppression on malaria parasitemia and clinical episode on adults in Rural Uganda: a cohort study. Lancet. 2000;356:1051-1056.

7. Megh R. HIV/AIDS and poverty. 2001. Pp 1-6.

8. Prescott LM, Hardy MP, Klein JP. Microbiology. 2004. 4th Edition. McGraw Hill, New York.

9. Peter P. Keynote address on HIV/AIDS crisis in Sub- Saharan Africa. UNAIDS. 2001

10. UNAIDS and WHO. AIDS epidemic update December.UNAIDS/04.45E. Geneva: UNAIDS/WHO. 2004.

11. Drag-Spira, R, Lepage P, Dabis F. Prevention of infectious complications of paediatric HIV infection in Africa. AIDS. 2000;14(9):1091-9.

12. Chaisson RE. Infections due to encapsulated bacteria, Salmonella, Shigella and Campylobacter. Infect Dis Clin North Am. 1988;2(2):475-84.

13. Krasinski K. Bacterial Infections. In: Pizzo PA, ed. Pediatric AIDS. Baltimore: Williams & Wilkins, 1994;241-253.

14. Wilfert CM. Invasive Bacterial Infections in Children with HIV Infection. In: Pizzo PA, Wilfert CM, Eds. Pediatric AIDS. Baltimore: Lippincott Williams & Wilkins, 2000;117-124.

15. Wang M, Daniel FS, George AJ, David CH. Emerging Plasmid-Mediated Quinolone Resistance Associated with the qnr Gene in Klebsiella pneumoniae Clinical Isolates in the United States. Antimicrob Agents Chemother. 2004;48(4):1295-1299.

16. Yah SC, Enabulele IO, Eghafona NO, Udemezue OO. Prevalence of Pseudomonas in Burn Wounds at the University of Benin Teaching Hospital. Benin City, Nigeria. J. Expt.& Anat (JCEA). 2004;3(1):12-15.

17. Zenobia W, Olga S, Krzysztof C et al. gyra mutations in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa in a Silesian hospital in Poland. Polish Journal of Microbiology. 2005;54(3): 201-206.

18. Putnam SD, Riddle MS, Wierzba TF et al. Antimicrobial susceptibility trends among Escherichia coli and Shigella species isolated from rural Egyptian Paediatroc population with diarrhoea between 1995 and 2000. Clin Microbiol Infect. 2004;10:804-810.

19. Cheesbrough M. District Laboratory Practice Manual in Tropical Countries. Part 2. Cambridge University Press. 2000. Pp 178-179.

20. Cowan ST, Steel KJ. Manual for the identification of medical bacteriology. 1993. Cambridge University Press. London, New York, Rockville, Melbourne and Sydney.

21. Bauer AW, Kirby WM, Sherris JC. Antibiotics susceptibility testing by a standardized single disk method. Am. J. Clin. Patho. 1997;45:493-496.

22. National Committee for clinical laboratory standards. Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that Grow aerobically. 5th Edition. Approved Standard M7-A5. NCCLS, Wayne, P. A. 2000.

23. WHO Drug Information 199. Essential Drugs Guidelines for Antimicrobial Susceptibility Testing. 7:68-78.

24. Olukoya DO, Oni O. Plasmids profile analysis and antimicrobial susceptibility patterns of Shigella isolates from Nigeria. Epidemiol. Infect. 1990;105:59-64.

25. Miller HJH. Experiment in molecular genetics cold spring hanbor. New York. 1982.

26. Zhou C, Yang Y, Jong AY. Using mini plasmids DNA for sequencing double stranded template with sequenase. Biotechniques, 1990;8:172-173.

27. Meyers JA, Sanchez D, Elwell LP, Falkows. Simple Agarose gel electrophoretic method for the identification and characterization of plasmids deoxyribonuclease acid. J. Bacterial 1976;127:1529-1537.

28. Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant DNA plasmids. Nucleic Acids Res. 1979;7:1513-523.

29. Maschmeyer G, Braveny I. Review of the incidence and prognosis of Pseudomonas aeruginosa infections in cancer patients in the 1990s. Eur J Clin Microbiol Infect Dis. 2000;19:915–25.

30. George HT, John EE Jr., David G, Michael S, John GB. Bad Bugs need an update on the development pipeline from the antimicrobial availability Task Force of the Infectious Disease Society of America. IDSA- Clinical Infectious Diseases (CID). 2006;42:657-664.

31. Enabulele OI, Ogbimi AO, Obuekwe CO. Aerobic bacteria in infected wounds. Nig. J. Microbiol. 1993;5: 171-82.

32. Yah SC, Enabulele IO, Eghafona NO. Bacteriological studies on infected Kerosene burn wounds in Benin City, Nigeria. Journal of Bio-Medical Investigation (JBI). 2004;2(1):4-9.

33. Obasiki-Obor EE, Salami CE. Susceptibility of urethritis Escherichia coli, Klebsiella species and Proteus species to antibiotics in Benin City. Nig. J. Microbio. 1983;3:114-120.

34. Olukoya DK, Olasupo NA. Drug resistance and plasmids profiles of Diarrhoeagenic bacteria isolates in Nigeria (1988-1996) Nig Qt. J. Hosp. Med 1997;7:26- 32.

35. Aluyi HSA, Arkortha EE. Plasmid profile of some enteric bacteria of diarrhoeal origin. Afri. Journal of Genetics. 2000;13:1-8.

36. Ling TKW, Jianhui X, Yunsong Y, Ching CL, Huifen Y, Peter MH, The MK0826 China Study Group. Multicenter Antimicrobial Susceptibility Survey of Gram-Negative Bacteria Isolated from Patients with Community-Acquired Infections in the People’s Republic of China. Antimicrobial Agents and Chemotherapy. 2006;50(1):374–378.

37. Sheikh AR, Afsheen A, Sadia K, Abdu W. Plasmid borne antibiotics resistance factors among indigenous Klebsiella. Pak J. Bot 2003;35(2):243-248.

38. Pearson CA. The role of district hospitals and the action in international medicine network. Infect Dis Clin North Am. 1995;9:391-405.

39. Okeke IN, Adebayo L, Edelman R. Socioeconomic and behavioral factors leading to acquire bacterial resistance to antibiotics in developing countries. Center For Disease Control and Prevention. Atlanta. GA USA. 1999

40. Thamlikitkul V. Antibiotic dispensing by drug store personnel in Bangkok, Thailand. J Antimicrob Chemother. 1988;21:125-31.

41. Kigotho AW. Ugandan doctors request antibiotic moratorium. Lancet. 1997;350:1014.

42. Dua V, Kunin CM, White LV. The use of antimicrobial drugs in Nagpur, India. A window on medical care in a developing country. Soc Sci Med. 1994;38:717-24

43. Haak H. Pharmaceuticals in two Brazilian villages: lay practices and perceptions. Soc Sci Med. 1988;27:1415-27.

44. Kafle KK, Gartoulla RP, Pradhan YM et al. Drug retailer training: experiences from Nepal. Soc Sci Med. 1992;35:1015-25.

45. Rahman F, Andersson R, Svanstrom L. Medical help seeking behaviour of injury patients in a community in Bangladesh. Public Health. 1998;112:31-5.

46. Fagbule D, Kalu A. Case management by community health workers of children with acute respiratory infections: implications for national ARI control programme. J Trop Med Hyg; 1995;98:241-6.


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